CBD2 is an updated database for colorectal cancer (CRC) biomarkers, expanding the original collection to 1569 new biomarkers across DNA, RNA, protein, and more, plus data on non-viable biomarkers. It features network analysis for exploring biomarker relationships and supports queries for chemicals and drug combinations, aiding in polypharmacological treatment design. CBD2 is free to use and aims to enhance CRC research and treatment strategies.

This chapter explores drug-target interactions in modern drug discovery, highlighting a shift from one-bullet-one-target to a holistic network approach. It reviews data sources, similarity-based and machine learning algorithms, and computational tools for interaction prediction and network analysis. Emphasizing the integration of big data and complex networks, the chapter underscores the potential to enhance our understanding of drug molecules, targets, and interactions.